![]() ![]() Kathy also offers private and group spiritual direction, and works with couples to create interfaith wedding and blessing ceremonies. She has served as a religious educator with children and families for 15 years and is currently on the Faith Formation and Education staff at Trinity Church in New York City, where she is chaplain to Trinity Preschool's multifaith faculty and student body. Diane Berke, as well as in progressive Christian social ethics at Boston University and Boston College. Bozzuti-Jones background includes studies in Interfaith ministry and Interspiritual counseling from One Spirit Interfaith Seminary (NYC), under the direction of Rev. York City and involved in development of an interspiritual global curriculum for children. Bozzuti-Jones is an interfaith minister and interspiritual counselor affiliated with One Spirit Learning Alliance in New In 2016 the Light of Truth Center was selected to be a part of West Baltimore Rise.Rev. In April of 2015 Vaile Leonard was the Keynote speaker for Johns Hopkins SOURCE 10-year anniversary ceremony. Vaile Leonard won the Weinberg Community Impact Award in 2011 won the Baltimore Times Positive People Award. In 2010 won the Ben Cardin Community Service Award. In 2014, LTC again achieved the illustrious Seal of Excellence from the Maryland Non-Profit organization. was awarded the MANO Seal of Excellence for 2010. After several years of working on the Maryland Association of Nonprofit Organizations Seal of Excellence Program (MANO), The Light of Truth Center Inc. In addition to the permanent supportive housing unit LTC has secured an additional office building which will provide a place for treatment, training and employment of our women in recovery. building which houses an additional eleven women and includes a library, laundry, computer lab, study and hairdresser. In 2008, LTC began a process to expand services and has successfully secured a 4032 square ft. She was awarded the woman of the year in 2005 by Live Your Life on Purpose and was awarded a Humanitarian award in 2006 for Black Wall Street. She has been inducted as an honorary core member at One God One Thought Center for Better Living for her continued service with the multi-media department and the Family Recovery Ministry. In 2010 she graduated from One Spirit Learning Alliance after an additional two years of study as an Interspiritual Counselor. She completed ministerial training at One Spirit Learning Alliance in New York City and was ordained as an Interfaith Minister in 2008. She completed and graduated from the Johnnie Coleman Theological Seminary with a Master Certificate, 1997. She is a successful graduate of the Weinberg Fellows program and graduated in 2008. In 2008 LTC was selected by the Weinberg foundation for its leadership training program. Vaile Leonard has been in long-term recovery for over twenty-five years and is a trained recovery advocate and certified trainer. (LTC) was incorporated in 1999 and opened its doors in 2000. supportive housing for women recovering from drugs and alcohol in Baltimore, Maryland. Reverend Vaile Leonard is the founder and CEO of the Light of Truth Center, Inc. ![]()
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![]() (2) Apps that have an dedicated uninstaller: Head to the /Applications folder in Finder. Hold down the app icon until all the icons begin to jiggle, then click the delete button that appears in the upper left corner of the icon, and click Delete again in the pop-up dialog to execute the uninstallation. (1) Apps downloaded from the App Store: Click the Launchpad icon in the Dock, and search for the app in Launchpad interface. When uninstalling antivirus/security or malware software, the better option is to reboot your Mac into Safe Mode to prevent automatic launch, and then perform the uninstallation.Īdditionally, if Logitech Control Center 3.9.5 comes with a built-in uninstaller, or it was installed via the Mac App Store, there is an alternative way you can take to perform the uninstallation. To find out and kill related background process(es), you can run Activity Monitor (located in /Applications/Utilities folder) instead. To quit an app, the common way is to right click app icon on the Dock and choose Quit option. Why I cannot delete Logitech Control Center 3.9.5? Being prompted that “The item Logitech Control Center 3.9.5 can’t be moved to the Trash because it’s open”? This message occurs because Logitech Control Center 3.9.5 (or its background process) is still running. This act is irrevocable so you must do it with caution. Note that the moment you empty the Trash, the app will be permanently removed from your Mac. During the process, you may be prompted to enter an administrator’s password to make the deletion take effect. Or choose right click Trash icon, choose Empty Trash, and Empty Trash again in the pop-up dialog.īesides, you also can trash the app from the Launchpad interface. Step 5: Choose Finder > Empty Trash from the Menu bar.Step 4: If prompted, enter administrator password and hit OK to allow the change.Also, you can right-click/control click Logitech Control Center 3.9.5 icon and then choose Move to Trash option from the sub menu. Step 3: Search for Logitech Control Center 3.9.5 in the /Applications folder, then drag its icon to the Trash icon located at the end of the Dock, and drop it there.Step 2: Launch Finder on your Mac, and click Applications in the Finder sidebar.Step 1: Quit Logitech Control Center 3.9.5 as well as its related process(es) if they are still running.Here’s the regular steps to uninstall Logitech Control Center 3.9.5 on Mac: In most circumstances you can take the drag-and-drop way to delete Logitech Control Center 3.9.5 from your computer. Most Mac apps are self-contained, and the trash-to-delete option works on all versions of Mac OS X and later. Question 1: How can I uninstall Logitech Control Center 3.9.5 from my Mac? ![]() So, when you decide to uninstall Logitech Control Center 3.9.5 on Mac, you will need to tackle the following two questions. The trash-to-delete method may leave some junk files behind, resulting in incomplete uninstall. That means, if you simply trash the app and think the removal is done, you’re wrong. Additionally, some apps may create supporting files, caches, login files scattering around the system directory. General knowledge: Once installed, an app is typically stored in the /Applications directory, and the user preferences for how the app is configured are stored in ~/Library/Preferences directory. ![]() If you have no clue how to do it right, or have difficulty in getting rid of Logitech Control Center 3.9.5, the removal solutions provided in the post could be helpful. This page is about how to properly and thoroughly uninstall Logitech Control Center 3.9.5 from Mac. Removing applications on Mac is pretty straightforward for experienced users yet may be unfamiliar to newbies. Perfect Solutions to Uninstall Logitech Control Center 3.9.5 for Mac ![]() ![]() ![]() ![]() Step three: pick out the variables given in the equation. The value of acceleration due to gravity, g, can be found by dropping a steel ball bearing and measuring the time it takes to fall, however the the very. ![]() Step two: pick out the unknown the question is asking you to solve for and add it to a givens list.Step one: read the entire problem and determine if an object is in the air (and not being propelled like a rocket), if only being affected by gravity a=10 m/s 2 down should be part of your givens list.If you push a toy car across the floor, it moves in the direction you pushed it. The motion of a free falling object can be described by Newtons second law of motion, force (F) mass (m) times acceleration (a). Free Fall Acceleration Under the constant acceleration of gravity near the. Jose dropped a ball from a height of 15 m from rest, how fast was it going before it hit the ground? Acceleration and Gravity: What Is the Relationship Between the Mass of an Object and Its Acceleration During Free Fall Introduction The motion of an object is the result of all the different forces that are acting on the object. Free Essay: Acceleration Due to Gravity Introduction In this lab you will. Example Acceleration Due to Gravity Question: When you read a problem and the object is in the air as a projectile (just under the influence of gravity), the acceleration will be 9.8 m/s 2 down. In later units we will encounter air resistance but unless you are told otherwise assume an ideal condition with no air resistance. Attach the right angle clamp to a vertical support rod. Freefall does not mean it fell to get in the air but is only under the influence of gravity. Experiment P007: Acceleration due to Gravity (Free Fall Adapter) 1. Scientists describe the motion of an object in free fall by describing its velocity and acceleration. When you read a problem that has an object thrown, dropped, or rolled off a table the object will be in freefall. 1) moves toward the center of the Earth because of gravity. If that is your goal, we recommend using a Photogate and a Picket. Working Through an Acceleration Due to Gravity (Freefall Problem) Motion Detectors do not provide the best measurement of the acceleration due to gravity. ![]() ![]() ![]() You can find out how to inspect element on android by using Inspect and Edit HTML Live Application. Now you know how to inspect element on android in a very easy way. After you click View Source, you can now see the inspect element of the site you typed. Then, you can click View Source to see the inspect element from a site. After you have successfully downloaded it, you can open the HTML Viewer Application and you can type the site you want to see the inspect element. You can download the HTML Viewer Application for free at the Play Store. The next way to inspect element on android is to use the HTML Viewer Application. After you have made the necessary changes on the web page, tap the edit icon again to save the changes from the Inspect Element you made. Now you can make the changes you want on the web page. When the website is open, tap the edit icon in the upper right corner. Then, type the website or URL of the web page you want to inspect. Open the application then by default the Google page will appear. You can search for the Edit Website App in Playstore then install it on your Android device. If you want to know how to inspect element on Android, you can use the Edit Website App. How to Inspect Element in Google Chrome is also very easy, you just right-click then select the Inspect Element menu. But if you use Google Chrome on a PC, you can make changes to Inspect Element directly. However, you cannot apply changes directly but you can only copy the webpage code and make changes elsewhere. Then, you can copy the code and make changes as needed. ![]() See also 11 Best Mood Board Apps for Android and iOS Here are 4 ways on how to inspect element on android that you should know: If you use Android, there are several ways you can use to inspect element. Inspect element have many benefits so it is essential to know how to inspect element. 4 Ways on How to Inspect Element on Android Apparently, there are many benefits of inspect element so it is important to know how to inspect element. Inspect element can also be used to measure memory performance and CPU performance. In fact, web developers use the Inspect element for debugging Javascript, making it easier to see Javascript error messages on the console. Web developers also use inspect element to test responsibility and also measure speed on a web. Usually, those who use the inspect element feature are web developers because they can experiment with CSS code using the inspect element. Now you know what an inspect element is and what its functions are. ![]() The Benefits of Inspect Element for Web Developers You can also see what CSS code is used, how javascript works on the website, and so on. In addition, the Inspect Element is very useful when you want to see the design of a website or see what fonts are used on the website. With the inspect element, you can change the appearance of the website or web pages on your device. Or, Inspect Element can also be interpreted as a browser feature created to facilitate the development of a website. Inspect Element is a software that you can use to edit HTML code or CSS code from a blog design. Some people might not know what Inspect Element is. Using Developer Tools Available in Chrome and Firefox 4 Ways on How to Inspect Element on Android.The Benefits of Inspect Element for Web Developers. ![]() ![]() ![]() Marcy Mays – My Curse (1993)Īfrika Bambaataa & The Soul Sonic Force – Planet Rock (1982)Īfter Forever – Leaden Legacy (The Embrace That Smothers – Part I) (2000)Īfter Forever – Yield To Temptation (The Embrace That Smothers – Part III) (2000)Īfterpartees – Candy Color Wearin’ Weirdos (2015)Īgainst Me! – Transgender Dysphoria Blues (2014)Īgalloch – In The Shadow Of Our Pale Companion (2002)Īge Of Love – Age Of Love (Jam & Spoon Watch Out For Stella Radio Mix) (1992)Īgent Side Grinder – In From The Cold (2018)Īgnetha Fältskog – If I Thought You’d Ever Change Your Mind (2004)Īlamo Race Track – Black Cat John Brown (2006)Īlamo Race Track – The Northern Territory (2006)Īlan Parsons Project – Silence And I (1982)Īlan Parsons Project – The Fall Of The House Of Usher (1976)Īlan Parsons Project – The Gold Bug (1980)Īlan Parsons Project Ft. 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Sarah Bettens – Mob Wife (2017)Ī Flock Of Seagulls – I Ran (So Far Away) (1982)Ī Flock Of Seagulls – Space Age Love Song (1982)Ī Flock Of Seagulls – Wishing (If I Had A Photograph of You) (1982)Ī Homeboy, A Hippie & A Funki Dredd – Total Confusion (1990)Ī Perfect Circle – So Long, And Thanks For All The Fish (2018)Ī Place To Bury Strangers – I Lived My Life To Stand In The Shadow Of Your Heart (2009)Ī Place To Bury Strangers – Missing You (2008)Ī Tribe Called Quest – Check The Rhime (1991)Ī Tribe Called Quest – Electric Relaxation (Relax Yourself Girl) (1993)Ī Tribe Called Quest – We The People (2016)Ī$AP Rocky Ft. Elton John – Ghetto Gospel (2004)ģ0 Seconds To Mars – From Yesterday (2006)ģ0 Seconds To Mars – Kings And Queens (2009)ģ0 Seconds To Mars – The Kill (Bury Me) (2005)Ī Balladeer – They’ve Shut Down Marks & Spencer (2004)Ī Balladeer Ft. ![]() …And You Will Know Us By The Trail Of Dead – Another Morning Stoner (2002)ġ0,000 Maniacs – What’s The Matter Here? (1987)ġ0cc – Feel The Benefit (Pt. Per decennium: oldies (< 60) | sixties | seventies | eighties | nineties | zeroes | tens | twenties Stem hier! !$(.# – 9 Op taal: Nederlandstalig | Duits, Frans, Italiaans, Spaans & Portugees Tevens zijn er subcategorieën: Girlpower | Lage Landen (Nederland & België) | Vergeten Hitjes | Xmas ![]() Van !!! tot ZzZ, maar om het je gemakkelijker te maken kan je ook per muziekgenre en per decennium zoeken.Īmbient | Americana | Artpop | Blues | Country | Dance (Disco, House, Techno & Trance) | Dreampop & Shoegaze | Electro | Experimentele, Psychedelische & Garagerock | Gospel | Hiphop & Rap | Indie | Jazz & Fusion | Klassiek & Filmmuziek | Kleinkunst | Krautrock | Metal | New Wave & Synthpop | Pop | Progressieve & Symfonische rock | Punk & Grunge | Reggae & Ska | Rock (Brit, Classic & Hardrock) | Rock & Roll | Singer-Songwriters & Folk | Soul & R&B | World & Latin ![]() Lekker alfabetisch door de keuzelijst heen lopen? Het duurt wel even, want dit jaar heb je de keuze uit meer dan 8.000 liedjes. ![]() ![]() Users on Metacritic gave the Xbox version of Bulb Boy a 7.6/10. He ultimately gave the game a "great" rating of 8/10 concluding that while it may be short, it was "clearly lovingly put-together" and managed to "refreshingly" make horror "cute and fun, rather than take itself seriously". Positive mention was also given to the game's final boss, which Craddock felt "offered a slightly different experience that was put together nicely". Ryan Craddock of Nintendo Life, similarly gave the Nintendo Switch version of Bulb Boy a positive review, citing its "successful mixture of ideas" which "see you doing different things as you progress through the story." Craddock also praised its "simple" controls, noting that while the lack of touchscreen input in a point-and-click style game might seem like a "missed opportunity", it meant that players would have the same experience whether in handheld or TV mode. He also bemoaned one instance where a control option wasn't clear to him, causing a puzzle to stump him for the "wrong reason". 735 Courier Boy Icons Browse & download freeand premium 735 Courier Boy Icons in colored outline, line, flat, glyph, gradient, dualtone, isometric, doodle, rounded design styles for web or mobile (iOS and Android) design, marketing, or developer projects. A Anne Chen 14 followers More information BD boy Find this Pin and more on Canada by Anne Chen. In 2021 we have brought top cb backgrounds for picsart and photoshop. Of the few gripes Nation had with Bulb Boy, its short length was one of them. Bad boy text png for editing by mmp picuture Download 500+ CB background HD. The touch controls were similarly lauded, with Nation calling the game "easy to pick up" as a result. ![]() He also praised the music, feeling that it was "appropriately themed" and helped "reinforce what's on the screen nicely". He praised the game's artstyle, positively comparing it to the work of The Ren & Stimpy Show creator John Kricfalusi calling the visuals "a lot of fun". Writing for Nintendo World Report, Justin Nation gave the game an 8/10. dragon boat festival creative vector origami banner free png and psd. SECRETS - There are things in the house waiting to be revealed.ĭownload Obb and Copy '' folder to 'sdcard/Android/Obb/'īulb Boy Free Download More Bulb Boy Download Freeĭownload Obb and Copy ' aggregator Metacritic gave the Switch version of Bulb Boy a score of 75 out of 100, indicating "generally favorable reviews". little boys are playing with a boat and magnifying glass png free water vassel two children having fun. ![]() Watch the story unfold in animated clouds. Boy Smart Idea Light Bulb png image file has been added to the Boy category by Jamie Knorscheidt at size of 509303, 1280x1088 resolution, you can Download. Relive Bulb Boy's childhood memories to uncover the mystery of the story.īUBBLE SPEECH - No dialogue, just bubble speech. PLAYABLE FLASHBACKS - Play as other family characters. Not only does your head light up and allows you to survive electrical shocks, but it can also be unscrewed for use in strange places. Defeat the evil in all its forms and bring the light back.īULB'S TALENTS - Being a boy that is a bulb comes with many talents. And don't forget to save your Grandpa-raffin and Mothdog from the frightening darkness.ĭEFEAT THE MONSTERS -Explore the Bulb house, that is full of frightening creatures. Copyright 2017 - 2022 APKmodus All Rights Reserved APKmodus. Solve puzzles, defeat wicked monsters and reveal bulby's abilities to unfold a twisted tale. One gloomy night, Bulb Boy wakes suddenly from a frightening nightmare to discover that evil has. white green fresh forest flamingo theme wedding stage renderings. european romantic cherry blossom wedding effect. denoting human beings and certain animals, e.g. light bulb dragon ball bulb led bulb dragon ball bulb off goku dragon ball lamp bulb hanging bulb white bulb glowing bulb bulb lights. Find light in yourself!īulb Boy is an intuitive 2D point & click horror adventure about a boy with a glowing head (alright, it's a bulb.) inspired by Machinarium and Gobliiins. There are ten PNGs, the nine listed in the first column in (13). Gather the courage and use his glass head to save everything he loves. His family has disappeared and there are horrid monsters lurking in the shadows. ![]() One gloomy night, Bulb Boy wakes suddenly from a frightening nightmare to discover that evil has overshadowed the Bulbhouse. ![]() ![]() ![]() ![]() ![]() Speaking of which, I chuckled after watching this. In fact, it could make those situations very awkward, or at the least, change them in ways you might not like. It’s easy to see how it’s amazing for parents to capture all of the adorable things their kids are doing, or for skydivers and rock climbers who clearly don’t have their hands free and also happen to be having life changing experiences.Īnd yes, it’s probably helpful if you’re in Thailand and need directions or translation - but this might not be that great at a dinner party, or on a date, or watching a movie. I don’t think Glass is right for every situation. Joshua Topolsky acknowledged as much in his interesting Glass review over at The Verge: More importantly, privacy is obviously going to be a big concern with a product that can stream whatever the person is looking at straight to the web, without folks even realizing they’re being filmed. The problem with the Glass is that the general public outside the technophiles could object to this idea of using sci-fi eyewear while appearing to be interacting with the world.įor starters, ignoring your surroundings in order to focus on a display could be a disaster waiting to happen. I’m liking Google Glass a lot and can’t wait to see what Apple has up its sleeve on the wearable devices front. Check out the comic included past the fold and meet us in comments… Putting aside for a moment the fact that one is the real product (Glass) while the other is vaporware (at least for now), both are futuristic wearable computers we’re supposed to wear on us in public. Earlier today, they weighed in on the inevitable comparison between the iWatch and Google’s Glass. There is also a settings menu, accessible from the gear icon in the top right corner of the window where you can pause your video, choose your source camera, access help, and more. Make choices here to select an effect, make an adjustment, or zoom and ip your picture. My favorite bit: it’s a walkie-talkie with a direct line to Jony Ive’s secret laboratory, Dick Tracy style. The iGlasses window is composed of four main sections: Presets, Effects, Adjustments, and Zoom & Flip. Joy of Tech last month posted a pretty comical take on the iWatch rumors. ![]() ![]() Blood, Language, Mild Suggestive Themes, Use of Alcohol and Tobacco, Violence. Players must learn to master the character that best suits their playstyle, weighing up if they prefer fast darting blows, powerful measured strikes or area-of-effect abilities that disrupt their foes. The Legend of Heroes: Trails of Cold Steel. This weekend, for the first time, console players will also be able to enter the Blood of Heroes arenas on PS4 and PS5. Is Blood Of Heroes Coming To PS5 And PS4 The new PvP melee combat game Blood Of. There are a total of six you’ll need to track down and kill, one of which will be the Sage of the branch. ![]() Like all the other branches, the members of the Heroes Of The Cult will be found throughout your long journey in Assassins Creed: Odyssey. Hone your skills in a battle where the artistry of the blade is enhanced by powerful war sigils-and met with blistering counterspells. Is Blood Of Heroes Coming To PS5 And PS4 David Carcasole / August 30, 2021. The Heroes of the Cult is one of the seven branches within the Cult Of Kosmos. Players can experiment with six distinct heroes in the closed beta in both one-on-one and group combat modes. In Blood of Heroes, battles are fought in the arenas of a. Set in medieval Nordic arenas, fearsome warriors fight to the death in a quest for glory as players unleash an array of fighting styles and wield an arsenal of lethal weapons and deadly abilities. Blood of Heroes is a multiplayer combat arena video game developed and published by Vizor Games. Here is an overview of the game, via Vizor Games:īlood of Heroes pits the greatest heroes of an unforgiving borderline world against each other in intense player-versus-player combat. Official By Players Blood of Steel - PvE Lu Ban Academy World-Introduction of Blood of Steel Heroes executing action in Blood of Steel display Blood of Steel final closed test will begin on Sept. Sign-ups are available now at the official website. It was a successor to DC Heroes and used that games Mayfair Exponential Game System, or MEGS. A PC closed beta test will begin on May 4. Blood of Heroes is a superhero role-playing game published by Pulsar Games. Developer Vizor Games has announced Nordic player-versus-player combat arena game Blood of Heroes for PlayStation 5, Xbox Series, PlayStation 4, Xbox One, and PC. ![]() ![]() ![]() However, it seems that there are players who have been getting some issues. However, since it doesn’t require any third-party tools or cheats, it’s unlikely that someone would get banned for using it. Just like any other PC game, Tribes of Midgard also features controller support. Explore a Bright Norse Realm Discover dungeons, slay legendary beasts, and build anywhere as you explore formidable, procedurally-generated lands on your quest to take on Ragnark. Starting off with the tutorial in Tribes of Midgard will make players perhaps more confident than they should be.Most randomized maps seemed to feature resources littered all over the ground for the player to snatch, but their availability is staggered collect branches, grass, and the like to make a lumberaxe and pickaxe, then use those tools to gather resources like wood and iron to make. Also, be warned, that those use this bug might find their progress rolled back. Surthrive the Viking way in Tribes of Midgard, a multiplayer, action-survival RPG of gigantic proportions. Mythical creatures, deadly spirits, and gigantic brutes threaten to bring. So, instead of actually enjoying Tribes of Midgard, someone using this bug is just going to sit around in menus.įortunately, since the exploit is now in the wild, Norsfell and Gearbox can patch it out and put everyone back on a level playing field. Heads up, the Giants are coming Midgard is under siege from unwelcome visitors. Even with the free XP, it’s going to take a bit to get to the max level. Of course, this is a really lame way to play the game. Keep your fingers crossed that it’s a high day count server.Right now, the leveling glitch in Tribes of Midgard can be done using these steps: Since users can join games in progress, they can get a sizable chunk of XP for just entering and immediately abandoning a world. The procedurally generated world and the ability to play as a Viking chieftain are some of the game's most popular features. The game has received generally positive reviews and has been praised for its mechanics and gameplay. ![]() This amount increases for each day added to the count. Locate the executable file in your local folder and begin the launcher to install your desired game. Tribes of Midgard is an action role-playing survival video game that was released in 2021. Players get a bit of XP for simply joining a game. The leveling exploit in Tribes of Midgard depends on an oversight in the experience system. ![]() ![]() ![]() ( B) Heat map of normalized expression values for 2185 genes that are differentially expressed between basal NK cells and NKAE cell products. ( A) Unsupervised hierarchical clustering of NK cells using the 15,919 genes remaining after filtering. Gene expression profiles of basal NK cells and NKAE cell products. GMP-grade NK cells for clinical use can be obtained by using different starting cells and aAPC.ĬliniMACS Prodigy NK cell activation and expansion NK cell immunotherapy NKAE cells clinical-grade manufacturing. Clinical-grade manufactured NKAE cells complied with the specifications from the Spanish Regulatory Agency. Transcriptome profile showed to be different between basal NK cells and NKAE cells expanded with K562mbIL21-41BBL or K562mbIL15-41BBL. However, no differences in activation and cytotoxicity were found when using either NK cell source or activating cell line. The highest fold expansion and NK purity were achieved by using PBMC and K562mbIL21-41BBL cells. Obtaining NKAE cells from CD45RA + cells was feasible although PBMC yielded higher total cell numbers and NK cell purity than CD45RA + cells. NK MACS and TexMACs achieved the highest NK cell purity and lowest T cell contamination. ![]() Clinical-grade NKAE cells were manufactured in CliniMACS Prodigy. Fold increase, NK cell purity, activation status, cytotoxicity and transcriptome profile were analyzed. Activated and expanded NK cells (NKAE) were obtained by coculturing total peripheral blood mononuclear cells (PBMC) or CD45RA + cells with irradiated K562mbIL15-41BBL or K562mbIL21-41BBL. RPMI-1640, stem cell growth medium (SCGM), NK MACS and TexMACS were used as culture mediums. We report the optimization of an NK cell activation-expansion process and its validation on clinical-scale. Natural killer (NK) cells represent promising tools for cancer immunotherapy. 11 Pediatric Hemato-Oncology Department, La Paz University Hospital, 28046 Madrid, Spain.10 Hematology Department 12 de Octubre University Hospital, 28041 Madrid, Spain. ![]() 9 Hematology and Hemotherapy Department, La Paz University Hospital, 28046 Madrid, Spain.8 Experimental Therapies and Novel Biomarkers in Cancer, La Paz University Hospital Institute for Health Research-IdiPAZ, 28046 Madrid, Spain.7 Applications Department Miltenyi Biotec, 28223 Madrid, Spain.6 Advanced Therapy Medicinal Products Production Unit, Pediatric Hemato-Oncology Service and Pharmacy Service, La Paz University Hospital, 28046 Madrid, Spain.5 Advanced Therapy Medicinal Products Production Unit Pediatric Hemato-Oncology Department, La Paz University Hospital, 28046 Madrid, Spain.4 Translational Research in Pediatric Oncology, Hematopoietic Transplantation & Cell Therapy, La Paz University Hospital Institute for Health Research-Institute of Medical and Molecular Genetics (INGEMM-IdiPAZ), 28046 Madrid, Spain. ![]() 3 Institute of Medical and Molecular Genetics (INGEMM), La Paz University Hospital, 28046 Madrid, Spain.2 Translational Research Group in Paediatric Oncology Haematopoietic Transplantation & Cell Therapy, La Paz University Hospital Institute for Health Research-IdiPAZ, 28046 Madrid, Spain.1 Hematological Malignancies Lab-H12O Clinical Research Unit, Spanish National Cancer Research Centre (CNIO), 28029 Madrid, Spain. ![]() |